Laboratory Techniques

The following are common procedures undertaken in a soilborne disease laboratory.

Media

• General Purpose Media
• Selective Media
• Media for Natural Inoculum

Sterilisation Techniques

• Heat
• Dry Heat
• Moist Heat
• Filtration
• Sterilisation of Instruments
• Sterilisation of Work Surfaces

Culturing Procedures

• Incubation
• Single-spore Transfer
• Hyphal Tip Transfer
• Degenerate Cultures
• Culture Mites

Preservation of Cultures

• Lyophilisation
• Silica Gel
• Soil Storage
• Water Storage
• Temporary Preservation
• Preservation for Herbarium Records

Isolation Procedures

• Isolation from Plants
• Soil Dilution Plates
• Debris Isolation
• Physical Separation of Particles
• Baiting

Pathogenicity Tests

Root Staining Procedures

• Mycorrhizae and Fungi
• Nematodes