Botanic Gardens Trust, Sydney, Australia

Laboratory Techniques

The following are common procedures undertaken in a soilborne disease laboratory.

Media

  • General Purpose Media
  • Selective Media
  • Media for Natural Inoculum

Sterilisation Techniques

  • Heat
  • Dry Heat
  • Moist Heat
  • Filtration
  • Sterilisation of Instruments
  • Sterilisation of Work Surfaces

Culturing Procedures

  • Incubation
  • Single-spore Transfer
  • Hyphal Tip Transfer
  • Degenerate Cultures
  • Culture Mites

Preservation of Cultures

  • Lyophilisation
  • Silica Gel
  • Soil Storage
  • Water Storage
  • Temporary Preservation
  • Preservation for Herbarium Records

Isolation Procedures

  • Isolation from Plants
  • Soil Dilution Plates
  • Debris Isolation
  • Physical Separation of Particles
  • Baiting

Pathogenicity Tests

Root Staining Procedures

  • Mycorrhizae and Fungi
  • Nematodes
   Nematode infection 
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